- For purified peptide libraries, we offer three purity levels at the customer’s discretion: >70%, >80, and up to 95%
- Peptide sequences may range in length from 3–20 amino acids
- Minimum order size of 24 peptides
- Delivery in 2–3 weeks
- Crude libraries come with a mass spec for each peptide
- Purified libraries come with mass spec and HPLC analysis for each peptide
Overlapping Peptide Library: Overlapping peptide libraries can be used for epitope mapping (linear and continuous), which can in turn be used to determine essential regions of a protein/ peptide contributing to the bioactivity. The library is designed based on two parameters: Peptide fragment length, and offset number. The peptide fragment length is the length of each peptide to be generated in the library, and is typically between 10 and 25 amino acids in length. The offset number is the degree of overlap with regard to the following peptide fragment.
Truncation Peptide Library: Truncated peptide libraries allow researchers to determine the minimum length of a peptide that is required for epitope activity. The original peptide sequence is systematically truncated, and each resulting peptide can then be tested. For long peptides, this can be used in conjunction with Inert insertion scanning libraries.
Alanine Peptide Scanning Library: Inert insertion scanning libraries, also known as alanine scanning libraries, are composed of a series of peptides in which one residue of the peptide backbone is systematically replaced with an alanine residue. The small methyl side chain of the alanine residue has relatively little biological activity and so a decrease in epitope activity is observed when an essential amino acid is replaced. In instances where size preservation is important, the amino acid may be replaced by a valine or leucine residue instead of an alanine residue.
Random Peptide Library: These libraries are synthesized by adding a mixture of all 20 natural amino acids into a specific position. This type of library may enhance peptide activity.
Scrambled Peptide Library: Scrambled libraries are composed of sequences containing (all) permutations of a given peptide sequence. This aids in peptide sequence optimization, and can be used to probe target molecules.
Positional Peptide Library: Positional importance scanning libraries are important in determining the optimum peptide sequence. It substitutes the amino acid(s) at positions of interest with all other natural amino acids sequentially. Using this methodology, it is possible to generate a more active peptide by identifying potentially more favourable residues for a given position.